Rapid and Efficient Regeneration of Rhododendron decorum from Flower Buds

Rhododendron decorum is a woody species with high ornamental and medical value. Herein, we introduce novel in vitro regeneration method for R. decorum. We used flower buds to develop an efficient rapid plant protocol. Sterile of 2 cm size were as explants study the effects culture medium growth regulators on callus induction adventitious shoot differentiation, proliferation, rooting. According results, optimal combination was WPM + 1 mg/L TDZ 0.2 NAA, its rate reached 95.08%. The differentiation from 0.5 0.1 91.32%. proliferation ZT which 95.32% coefficient 9.45. rooting shoots NAA IBA, 86.90%. survival rates rooted regenerated plantlets exceeded 90% after acclimatization transplantation. This system has advantages being simple highly efficient, it causes little damage mother plants, laying foundation plantlet propagation, genetic transformation, new-variety breeding